Ebumnuche [Abstract] Iji chọpụta ihe fọdụrụ amino acid N-terminal 15 nke akụkụ abụọ yiri nke Agacutin.Ọ dị mkpa ka ekewa akụkụ abụọ nke molekul enzyme ahụ iji nweta otu n'ime otu peptide yinye na usoro.Ụzọ ndị Agacutin sachara nke ọma bụ nke enwetara site na usoro biochemical.A kwadebere ihe nlele otu mpaghara maka njirimara na usoro site na SDS-PAGE electrophoresis separation, mgbake nke ntanye ntanye na teknụzụ PVDF membrane electrotransfer.Nke ikpeazụ kpebiri mfu amino acid iri na ise na N-terminal nke subunit site na usoro mmebi iwu Edman.Nsonaazụ Usoro nke nnukwu subunit (16 kDa) bụ DCSSGWSSYEEHQYY, na nke obere obere obere (15 kDa) bụ DSSGWSSYEGHEYYV.Mmechi Nsonaazụ nke usoro gosipụtara na Agacutin bụ thrombin agwọ ọhụrụ dị ka enzyme site na ịchọ nchekwa data NCBI.
Oge nzipu: Dec-22-2022