Venom agwọ thrombin dị ka enzyme (TLE) bụ serine protease nke ezinụlọ trypsin, nke nwere ọtụtụ usoro echekwara na trypsin.Ọ nwere ọrụ arginine esterase, nwere ike na-eme ihe ozugbo na fibrinogen, na-eme ka nkwụsị nke Arg2Gly peptide bond na akụkụ ụfọdụ nke fibrinogen molecule, hapụ fibrinopeptide A (FPA) ma ọ bụ B (FPB. A na-ekewa thrombins ole na ole alkaline site na kọlụm mgbanwe cation, jikọtara ya na ya. gel filtration na affinity chromatography Affinity chromatography na-eji ọtụtụ ebe n'ihi na ya dị elu nkewa arụmọrụ na elu sample mgbake. P3 na P4 sitere na venom nke Agkistrodon acutus na Fujian site na iji usoro dị ọcha dị iche iche. Nnyocha na-egosi na ha abụọ nwere ọrụ thrombin, mana EDTA na-egbochi ọrụ thrombin nke akụkụ P4 kpamkpam ma gbochie ọrụ coagulase nke P3. Component P4 enweghị. ọrụ nke ịgbalite ihe
Oge nzipu: Dec-23-2022