Nkwadebe na ọmụmụ nke IgY Antibodies megide ihe ndabere nke Metallases sitere na Agkistrodon halys Venom

[Akụkụ] [Ebumnobi] Iji kwadebe ọgwụ mgbochi IgY megide ebumnuche nkịtị nke metalloproteinases sitere na Agkistrodon acutus venom, yana mụọ mmetụta ya na ọrụ venom agwọ [Usoro] Site n'ịtụle usoro nke metalloproteinases dị iche iche sitere na Agkistrodon acutus venom, anyị haziri. na synthesized nke ukwuu antigen peptides PT1 na PT2, nke nwere njikọ chiri anya na ọrụ enzyme;A gbara ọkụkọ ọgwụ mgbochi site na ngwakọta njikọ KLH-PT1 na KLH-PT2 iji nweta ọgwụ mgbochi IgY.ELISA na Western blot mụrụ Titer nke IgY na njirimara mmeghachi omume na Agkistrodon acutus na Agkistrodon acutus venom;N'ikpeazụ, a na-enyocha ọrụ nkwụsị nke IgY site na nyocha ọbara ọgbụgba nke subcutaneous na ụmụ oke [Nsonaazụ] Nsonaazụ ELISA na Western blot gosiri na mgbochi KLH-PT1 na anti-Agkistrodon acutus IgY nwere ike ịgafe na Agkistrodon acutus na Agkistrodon acutus venom , na nke ikpeazụ dị nnọọ ike karịa nke mbụ;Anti-KLH-PT2 IgY enweghị mmeghachi omume n'ụzọ doro anya na Agkistrodon acutus venom na Agkistrodon acutus venom in vitro, mana ọ nwere nsi na-ewepụ nsí siri ike na nnwale mgbochi ọbara na vivo, yana ọ dị elu karịa nke abụọ mbụ [Nkwubi okwu] The IgY A kwadoro ọgwụ mgbochi nke nwere ike ịgafe n'ọbara dị iche iche na-ekesa venoms agwọ site n'ichepụta usoro antigen peptide nke metalloproteinase, bụ nke tọrọ ntọala maka nkwadebe nke kpọmkwem, ịrụ ọrụ nke ọma, obere toxicity na nnukwu ụdịdị dị iche iche mgbochi hemorrhagic agwọ venom antibody.